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Enzyme Information

Protease 3.0

I.U.B. 9025-49-4


Proteases include both endo-peptidases and exo-peptidases. Endo-peptidases break the internal bonds of a protein and produce shorter peptide fragments. Exo-peptidases break the terminal bonds and release free amino acids. These proteases work synergistically with endogenous enzymes to help digest the proteins found in meats, eggs, cheese, legumes, nuts and other food

The food grade acid-stable protease enzyme used in National Enzyme Company products is obtained by the controlled fermentation of Aspergillus niger. This protease is characterized by its ability to hydrolyze proteins under acid conditions. The broad specificity of acid- stable protease enables the enzyme to, easily and efficiently, hydrolyze most soluble proteins.


The activity of acid-stable protease is measured in the FCC Spectrophotometric Acid Protease Units (SAPU), the assay of which is based on a 30-minute hydrolysis of a Hammarstan Casein Substrate at pH 3.0 and 37°C. One SAPU of proteolytic activity is defined as that amount of enzyme that will liberate one micromole of tyrosine per minute under conditions of the assay.


This enzyme is used in the production of soluble proteins in such diverse industries as brewing and photographic films. Within the health and wellness industry, proteolytic enzymes have been shown to provide therapeutic action when used in cardiovascular support, joint support, and muscle support formulas and as digestive aids.

Shelf Life:

To maintain optimum enzyme activity, this product should be stored in a cool, dry place in a tightly sealed container. When properly stored, this product can be expected to lose less than 10% of its activity in twelve months.

Typical Side Activities:

A variety of proteolytic activities


  • Form: Dry powder
  • Color: Light tan
  • Odor: Free of offensive odor
  • Taste: Free of offensive taste
  • Effective pH Range: pH 2.75 to 4.7
  • Optimum pH: pH 3.0 to 3.5
  • Effective Temperature Range:Up to 55°C
  • Optimum Temperature: 40°C

Nothing disclosed above is to be construed as a recommendation to use our product in violation of any patents. The information presented above is believed to be accurate. However, said information and products are offered without warranty or guarantee except as to the composition and purity stated herein since the ultimate conditions of use and variability of the materials treated are beyond our control.

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