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Enzyme Information

Peptidase

I.U.B. 3.4.11.1

Description:

Proteases include both endo-peptidases and exo-peptidases. Endo-peptidases break the internal bonds of a protein and produce shorter peptide fragments. Exo-peptidases break the terminal bonds and release free amino acids. These proteases work synergistically with endogenous enzymes to help digest the proteins found in meats, eggs, cheese, legumes, nuts and other foods.

The food grade peptidase enzyme used in National Enzyme Company products is obtained by the controlled fermentation of Aspergillus oryzae. This enzyme system contains a very high level of exopeptidase activity. The exo-peptidases liberate amino acids by hydrolysis of the peptide bonds at the terminus of the peptide chain. Peptidase FP provides both aminopeptidase and carboxypeptidase activity. The high exo-peptidase level allows the user to obtain a high degree of protein hydrolysis without formation of bitter products.

Activity:

The activity of the protease enzymes is measured in the FCC Hemoglobin Unit on a Tyrosine Basis (HUT), the assay of which is based on the hydrolysis of denatured hemoglobin. One HUT unit of proteolytic (protease) activity is defined as that amount of enzyme that produces, in one minute under the specified conditions, a hydrolysate whose absorbance at 275nm is the same as that of a solution containing 1.10 μg per ml of tyrosine in 0.006N hydrochloric acid.

Applications:

Within the food industry, proteolytic enzymes are used to produce protein hydrosylates and as meat tenderizers. They have commercial applications in leather production, the processing of raw silk and detergent manufacturing. Within the health and wellness industry, proteolytic enzymes have also been shown to possess valuable therapeutic action as anti-inflammatory agents and digestive aids.

Shelf Life:

To maintain optimum enzyme activity, this product should be stored in a cool, dry place in a tightly sealed container. When properly stored, this product can be expected to lose less than 10% of its activity in twelve months.

Typical Side Activities:

A variety of proteolytic activities

Properties:

  • Form: Dry powder
  • Color: Light tan
  • Odor: Free of offensive odor
  • Taste: Free of offensive taste
  • Effective pH Range: pH 3.25 to 7.5
  • Optimum pH: pH 4.7 to 6.25
  • Effective Temperature Range:Up to 60°C
  • Optimum Temperature: 50°C to 60°C
Note:

Nothing disclosed above is to be construed as a recommendation to use our product in violation of any patents. The information presented above is believed to be accurate. However, said information and products are offered without warranty or guarantee except as to the composition and purity stated herein since the ultimate conditions of use and variability of the materials treated are beyond our control.

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