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Enzyme Information


I.U.B. 9001-73-4


The papain used in National Enzyme Company products is a food grade mixture of proteolytic enzymes isolated from the fruit of the tropical plant, Carica papaya. Papain contains a wide array of proteolytic enzymes, incorporating a broad range of substrate specificity and optimum environments. Because of this attribute, papain easily and efficiently hydrolyzes most soluble protein, yielding peptides and amino acids.


The activity of the papain is measured in the FCC Papain Unit (PU), the assay of which is based on the hydrolysis of casein. This assay is based on a 60-minute proteolytic hydrolysis of a casein substrate at pH 6.0 and 40°C. One PU is defined as that quantity of enzyme that liberates the equivalent of 1 g of tyrosine per hour under the conditions of the assay.


Within the food industry, proteolytic enzymes are used to produce protein hydrosylates and as meat tenderizers. Within the health and wellness industry, proteolytic enzymes have been shown to provide therapeutic action when used in cardiovascular support, joint support, and muscle support formulas and as digestive aids.

Shelf Life:

To maintain optimum enzyme activity, this product should be stored in a cool, dry place in a tightly sealed container. When properly stored, this product can be expected to lose less than 10% of its activity in twelve months.

Typical Side Activities:

A variety of proteolytic activities


  • Form: Dry powder
  • Color: Light tan
  • Odor: Pungent and somewhat offensive
  • Taste: Unpleasant
  • Effective pH Range: pH 3.0 to 10.0
  • Optimum pH: pH 5.0 to 7.0
  • Effective Temperature Range:65°C to 80°C
  • Optimum Temperature: 65°C

Nothing disclosed above is to be construed as a recommendation to use our product in violation of any patents. The information presented above is believed to be accurate. However, said information and products are offered without warranty or guarantee except as to the composition and purity stated herein since the ultimate conditions of use and variability of the materials treated are beyond our control.