Search Enzymes

Enzyme Information

Neutral Bacterial Protease

I.U.B. 9068-59-1


Proteases include both endo-peptidases and exo-peptidases. Endo-peptidases break the internal bonds of a protein and produce shorter peptide fragments. Exo-peptidases break the terminal bonds and release free amino acids. These proteases work synergistically with endogenous enzymes to help digest the proteins found in meats, eggs, cheese, legumes, nuts and other foods.

The food grade neutral bacterial protease enzyme used in National Enzyme Company products is obtained by the controlled fermentation of Bacillus subtilus. Neutral bacterial protease is an endopetidase that hydrolyzes the interior bonds of proteins. This protease easily and efficiently hydrolyzes most soluble proteins.


The activity of neutral bacterial protease is measured in the FCC PC unity. One PC unit is defined as the quantity of enzyme that produces the equivalent of 1.5 μg per ml of L-tyrosine per minute under conditions of the assay. The assay is based on a 30-minute hydrolysis of casein at 37°C and pH 7.0.


This enzyme is used in the production of soluble proteins in such diverse industries as brewing and agriculture. Within the health and wellness industry, proteolytic enzymes have been shown to provide therapeutic action when used in cardiovascular support, joint support, and muscle support formulas and as digestive aids.

Shelf Life:

To maintain optimum enzyme activity, this product should be stored in a cool, dry place in a tightly sealed container. When properly stored, this product can be expected to lose less than 10% of its activity in twelve months.

Typical Side Activities:

A variety of proteolytic activities


  • Form: Dry powder
  • Color: Light tan
  • Odor: Free of offensive odor
  • Taste: Free of offensive taste
  • Effective pH Range: pH 6.5 to 8.5
  • Optimum pH: pH 6.5 to 7.0
  • Effective Temperature Range:10°C to 70°C
  • Optimum Temperature: 55°C

Nothing disclosed above is to be construed as a recommendation to use our product in violation of any patents. The information presented above is believed to be accurate. However, said information and products are offered without warranty or guarantee except as to the composition and purity stated herein since the ultimate conditions of use and variability of the materials treated are beyond our control.

Technical Data Sheet Download

PDF Download Download PDF