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Enzyme Information


I.U.B. 9001-63-2


The food grade lysozyme enzyme used in National Enzyme Company products is a white crystalline powder of lysozyme hydrochloride (N-acetylhexosaminodase), extracted from hen egg albumen. Lysozyme catalyzes the hydrolysis of β-1,4-glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine, which occur in the cell walls of certain microorganisms.


The activity of lysozyme is based on the rate of lysis of a suspension of Micrococcus lysodeikticus compared to a reference standard. Lysis is measured spectrophotometrically at 540 nm, pH 6.6 and 37°C (98.6°F). Lysozyme has optimum activity in the pH range 3.5 to 7.0 and has a guaranteed minimum activity of 90 percent.


Lysozyme is used for the hydrolysis of microbial cells and release of their contents. Food applications include the prevention of late blowing of semi-hard cheeses due to the growth of Clostridium tyrobutyricum.

Shelf Life:

When refrigerated and kept dry, the declared activity of lysozyme is maintained for one year. However, it can be shipped without refrigeration.


  • Form: Dry powder
  • Color: Off-white
  • Odor: Free of offensive odor
  • Taste: Free of offensive taste
  • Effective pH Range: pH 3.5 to 7.0
  • Optimum pH: pH 6.6
  • Effective Temperature Range:30°C to 90°C
  • Optimum Temperature: 55°C

Other Characteristics:

The temperature stability of lysozyme depends upon pH; the enzyme is more stable at acidic than at alkaline pH. For example, under acidic conditions the enzyme is stable to 100°C (212°F); however, in an alkaline medium, it quickly loses lytic activity as temperature rises.

Isoelectric point: pH 10.7
Activity inhibitors: Sodium dodecyl sulfate; alcohols; imidazole; indole derivatives; Nacetyl-D-glucosamine EDTA; amino acids; organic acids; phytic acid; hydrogen peroxide
Moisture: 6 percent
Solubility: Freely soluble in water
pH (2% solution): pH 3.0 to 4.0 at 25°C


Nothing disclosed above is to be construed as a recommendation to use our product in violation of any patents. The information presented above is believed to be accurate. However, said information and products are offered without warranty or guarantee except as to the composition and purity stated herein since the ultimate conditions of use and variability of the materials treated are beyond our control.

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