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Enzyme Information

Cellulase

I.U.B. 3.2.1.4C.A.S. 9012-54-8

Description:

Fiber-breaking enzymes are capable of hydrolyzing the nondigestible carbohydrates that comprise fiber. The human body does not produce enzymes to break down fiber, but instead is at the mercy of enzymes produced by intestinal microorganisms. While fiber is an important part of a healthy diet, it may also act as an anti-nutritive factor. Fiber may bind to minerals and other nutrients, blocking their bioavailability and preventing their absorption.

The cellulase enzyme used by National Enzyme Company is a food grade cellulase complex obtained by the controlled fermentation of Trichoderma longibrachiatum. It hydrolyzes the beta‐D‐1,4‐ glucosidic bonds of cellulose, its oligomers and derivatives. This enzyme is a complex composed of three distinct enzymes to convert cellulose to glucose. One component serves to weaken the structure of native cellulose by weakening the hydrogen bonds. A second component consists of exo and endo‐beta‐ 1,4‐ glucanases. The exo‐glucanase removes single glucose units from the non‐reducing end of the cellulose chain, while the endo‐glucanase hydrolyzes the interior glucosidic bonds of cellulose to liberate oligomers of lower molecular weight. A third component consists of the betaglucosidases, including cellobiase, which are active on the dimers and oligomers of cellulose.

Activity:

One FCC Cellulase Unit (CU) is that activity that will produce a relative fluidity change of one in five minutes in a defined carboxymethyl cellulose substrate under the conditions of the assay. This assay is based on the enzymatic hydrolysis of the interior beta‐1,4‐glucosidic bonds of a defined carboxymethyl cellulose substrate at pH 4.5 and 40°C. The corresponding reduction in substrate viscosity is determined with a calibrated viscometer.

Applications:

This enzyme’s primary use is as a digestive aid, particularly in animals. Cellulase is also used to produce glucose syrups and in paper milling. Research is going on to determine the possible use of this enzyme in the treatment of wastes. The end product of this reaction is glucose.

Shelf Life:

To maintain optimum enzyme activity, this product should be stored in a cool, dry place in a tightly sealed container. When properly stored, this product can be expected to lose less than 10% of its activity in twelve months.

Typical Side Activities:

Hemicellulase and xylanase

Properties:

  • Form: Dry powder
  • Color: Light tan
  • Odor: Free of offensive odor
  • Taste: Free of offensive taste
  • Effective pH Range: pH 2.0 to 8.0
  • Optimum pH: pH 4.0 to 6.0
  • Effective Temperature Range:Up to 60°C
  • Optimum Temperature: 55°C
Note:

Nothing disclosed above is to be construed as a recommendation to use our product in violation of any patents. The information presented above is believed to be accurate. However, said information and products are offered without warranty or guarantee except as to the composition and purity stated herein since the ultimate conditions of use and variability of the materials treated are beyond our control.

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