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Enzyme Information


I.U.B. 37189-34-7


The proteolytic fraction from the pineapple has a long history of use for immune support.

The food grade bromelain enzyme used in National Enzyme Company products is a mixture of enzymes isolated from both the ripe and unripe fruit as well as the stem of the pineapple plant, Ananas comosus. Bromelain contains several proteolytic enzymes that differ in their specificity and optimum environments. Bromelain breaks down proteins to form peptides and amino acids with preferential cleavage of peptide bonds where the carbonyl group is from either a basic amino acid or an aromatic amino acid.


The activity of bromelain is measured in the FCC PU, the assay of which is based on the hydrolysis of casein. This assay is based on a 60-minute proteolytic hydrolysis of a casein substrate at pH 6.0 and 40°C. One PU (Papain Unit) is defined as that quantity of enzyme that liberates the equivalent of 1 g of tyrosine per hour under the conditions of the assay.


Within the food industry, proteolytic enzymes are used as meat tenderizers and to produce protein hydrosylates. They have commercial applications in leather production, the processing of raw silk and detergent manufacturing. Within the health and wellness industry, proteolytic enzymes have been shown to provide therapeutic action when used in cardiovascular support, joint support, and muscle support formulas and as digestive aids.

Shelf Life:

To maintain optimum enzyme activity, this product should be stored in a cool, dry place in a tightly sealed container. When properly stored, this product can be expected to lose less than 10% of its activity in twelve months.

Typical Side Activities:

A variety of proteolytic activities


  • Form: Dry powder
  • Color: Light tan
  • Odor: Free of offensive odor
  • Taste: Free of offensive taste
  • Effective pH Range: pH 4.0 to 9.0
  • Optimum pH: pH 5.0 to 8.0
  • Effective Temperature Range:40°C to 65°C
  • Optimum Temperature: 55°C

Nothing disclosed above is to be construed as a recommendation to use our product in violation of any patents. The information presented above is believed to be accurate. However, said information and products are offered without warranty or guarantee except as to the composition and purity stated herein since the ultimate conditions of use and variability of the materials treated are beyond our control.

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